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Optical Density is used as a measurement of the amount of bacteria growing in the culture medium
The more bacteria the cloudier the mixture and the higher the OD
When the bacteria is multiplying
Stationary phase
Replicative phase
Lag phase
Amount of bacteria in culture
When replication stops
due to overcrowding and they start to manufacture products
Time for the bacteria to
“wake up"
BacLyte
reduces lag
phase
With BacLyte added to culture
Wakes up
bacteria sooner
Stationary phase
occurs at a HIGHER OD
⦿ More bacteria in culture
⦿ More product produced
Time for the bacteria to
"Wake Up!"
Increases
rate of
replication
When the bacteria is Multiplying
End result = Production of
product starts sooner
Increase yield of product
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